All the best of luck with diagnosis, do test for ehrlichiosis, although both ehrlichiosis and leish can be difficult to get a definitive diagnosis. More info on Leish for those interested (I am sure it has been posted elsewhere in this forum): There is cutting edge research going on with Leish; Dr. Petersen at Iowa State University is at the forefront of said research. Her current study is looking at the prevalence of the parasite in the US in a number of breeds, and she has graciously decided to include the Neapolitan Mastiff in the study. More information is at: http://www.everythingneo.com/ under the medical section. My thoughts are with you and your dog. Cheers, Robin