[blockquote]There is a very small window of opportunity to get puppies out - day 61 is the usual date for Neapolitan Mastiffs to give birth - if not out by then you have 24 hours to do so. We have had bitches with large litters give birth at 57 and 58 days - fully formed puppies with no problems. [/blockquote] Days are counted from the DATE OF OVULATION, not the date of breeding. Ovulation date can only be determined accurately by hormone testing sent out to laboratory.